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Differential hERG ion channel activity of ultrasmall gold nanoparticles
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Zeitschriftentitel: | Proceedings of the National Academy of Sciences of the United States of America |
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Personen und Körperschaften: | , , , , , , , , , , |
In: | Proceedings of the National Academy of Sciences of the United States of America, 110, 2013, 20, S. 8004-8009 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
National Academy of Sciences
|
finc.format |
ElectronicArticle |
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finc.mega_collection |
sid-55-col-jstorlife sid-55-col-jstorhealth JSTOR Life Sciences Archive JSTOR Health & General Sciences |
finc.id |
ai-55-aHR0cHM6Ly93d3cuanN0b3Iub3JnL3N0YWJsZS80MjY1NjYyNg |
finc.source_id |
55 |
ris.type |
EJOUR |
rft.atitle |
Differential hERG ion channel activity of ultrasmall gold nanoparticles |
rft.epage |
8009 |
rft.genre |
article |
rft.issn |
0027-8424 |
rft.issue |
20 |
rft.jtitle |
Proceedings of the National Academy of Sciences of the United States of America |
rft.tpages |
5 |
rft.pages |
8004-8009 |
rft.pub |
National Academy of Sciences |
rft.date |
2013-05-14 |
x.date |
2013-05-14T00:00:00Z |
rft.spage |
8004 |
rft.volume |
110 |
abstract |
<p>Understanding the mechanism of toxicity of nanomaterials remains a challenge with respect to both mechanisms involved and product regulation. Here we show toxicity of ultrasmall gold nanoparticles (AuNPs). Depending on the ligand chemistry, 1.4-nm-diameter AuNPs failed electrophysiology-based safety testing using human embryonic kidney cell line 293 cells expressing human ether-á-go-go-Related gene (hERG), a Food and Drug Administration-established drug safety test. In patch-clamp experiments, phosphine-stabilized AuNPs irreversibly blocked hERG channels, whereas thiol-stabilized AuNPs of similar size had no effect in vitro, and neither particle blocked the channel in vivo. We conclude that safety regulations may need to be reevaluated and adapted to reflect the fact that the binding modality of surface functional groups becomes a relevant parameter for the design of nanoscale bioactive compounds.</p> |
authors |
Array
(
[rft.aulast] => Leifert
[rft.aufirst] => Annika
)
Array ( [rft.aulast] => Pan [rft.aufirst] => Yu ) Array ( [rft.aulast] => Kinkeldey [rft.aufirst] => Anne ) Array ( [rft.aulast] => Schiefer [rft.aufirst] => Frank ) Array ( [rft.aulast] => Setzler [rft.aufirst] => Julia ) Array ( [rft.aulast] => Scheel [rft.aufirst] => Olaf ) Array ( [rft.aulast] => Lichtenbeld [rft.aufirst] => Hera ) Array ( [rft.aulast] => Schmid [rft.aufirst] => Günter ) Array ( [rft.aulast] => Wenzel [rft.aufirst] => Wolfgang ) Array ( [rft.aulast] => Jahnen-Dechent [rft.aufirst] => Willi ) Array ( [rft.aulast] => Simon [rft.aufirst] => Ulrich ) |
languages |
eng |
url |
https://www.jstor.org/stable/42656626 |
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author | Leifert, Annika, Pan, Yu, Kinkeldey, Anne, Schiefer, Frank, Setzler, Julia, Scheel, Olaf, Lichtenbeld, Hera, Schmid, Günter, Wenzel, Wolfgang, Jahnen-Dechent, Willi, Simon, Ulrich |
author_facet | Leifert, Annika, Pan, Yu, Kinkeldey, Anne, Schiefer, Frank, Setzler, Julia, Scheel, Olaf, Lichtenbeld, Hera, Schmid, Günter, Wenzel, Wolfgang, Jahnen-Dechent, Willi, Simon, Ulrich, Leifert, Annika, Pan, Yu, Kinkeldey, Anne, Schiefer, Frank, Setzler, Julia, Scheel, Olaf, Lichtenbeld, Hera, Schmid, Günter, Wenzel, Wolfgang, Jahnen-Dechent, Willi, Simon, Ulrich |
author_sort | leifert, annika |
collection | sid-55-col-jstorlife, sid-55-col-jstorhealth |
container_issue | 20 |
container_start_page | 8004 |
container_title | Proceedings of the National Academy of Sciences of the United States of America |
container_volume | 110 |
description | <p>Understanding the mechanism of toxicity of nanomaterials remains a challenge with respect to both mechanisms involved and product regulation. Here we show toxicity of ultrasmall gold nanoparticles (AuNPs). Depending on the ligand chemistry, 1.4-nm-diameter AuNPs failed electrophysiology-based safety testing using human embryonic kidney cell line 293 cells expressing human ether-á-go-go-Related gene (hERG), a Food and Drug Administration-established drug safety test. In patch-clamp experiments, phosphine-stabilized AuNPs irreversibly blocked hERG channels, whereas thiol-stabilized AuNPs of similar size had no effect in vitro, and neither particle blocked the channel in vivo. We conclude that safety regulations may need to be reevaluated and adapted to reflect the fact that the binding modality of surface functional groups becomes a relevant parameter for the design of nanoscale bioactive compounds.</p> |
facet_avail | Online, Free |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-55-aHR0cHM6Ly93d3cuanN0b3Iub3JnL3N0YWJsZS80MjY1NjYyNg |
imprint | National Academy of Sciences, 2013 |
imprint_str_mv | National Academy of Sciences, 2013 |
institution | DE-D13, DE-15, DE-14 |
issn | 0027-8424 |
issn_str_mv | 0027-8424 |
language | English |
last_indexed | 2024-03-01T22:36:52.231Z |
match_str | leifert2013differentialhergionchannelactivityofultrasmallgoldnanoparticles |
mega_collection | JSTOR Life Sciences Archive, JSTOR Health & General Sciences |
physical | 8004-8009 |
publishDate | 2013 |
publishDateSort | 2013 |
publisher | National Academy of Sciences |
record_format | ai |
recordtype | ai |
score | 18,864033 |
series | Proceedings of the National Academy of Sciences of the United States of America |
source_id | 55 |
spelling | Leifert, Annika Pan, Yu Kinkeldey, Anne Schiefer, Frank Setzler, Julia Scheel, Olaf Lichtenbeld, Hera Schmid, Günter Wenzel, Wolfgang Jahnen-Dechent, Willi Simon, Ulrich 0027-8424 National Academy of Sciences https://www.jstor.org/stable/42656626 <p>Understanding the mechanism of toxicity of nanomaterials remains a challenge with respect to both mechanisms involved and product regulation. Here we show toxicity of ultrasmall gold nanoparticles (AuNPs). Depending on the ligand chemistry, 1.4-nm-diameter AuNPs failed electrophysiology-based safety testing using human embryonic kidney cell line 293 cells expressing human ether-á-go-go-Related gene (hERG), a Food and Drug Administration-established drug safety test. In patch-clamp experiments, phosphine-stabilized AuNPs irreversibly blocked hERG channels, whereas thiol-stabilized AuNPs of similar size had no effect in vitro, and neither particle blocked the channel in vivo. We conclude that safety regulations may need to be reevaluated and adapted to reflect the fact that the binding modality of surface functional groups becomes a relevant parameter for the design of nanoscale bioactive compounds.</p> Differential hERG ion channel activity of ultrasmall gold nanoparticles Proceedings of the National Academy of Sciences of the United States of America |
spellingShingle | Leifert, Annika, Pan, Yu, Kinkeldey, Anne, Schiefer, Frank, Setzler, Julia, Scheel, Olaf, Lichtenbeld, Hera, Schmid, Günter, Wenzel, Wolfgang, Jahnen-Dechent, Willi, Simon, Ulrich, Proceedings of the National Academy of Sciences of the United States of America, Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title_full | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title_fullStr | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title_full_unstemmed | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title_short | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
title_sort | differential herg ion channel activity of ultrasmall gold nanoparticles |
title_unstemmed | Differential hERG ion channel activity of ultrasmall gold nanoparticles |
url | https://www.jstor.org/stable/42656626 |